Cortisol is a glucocorticoid made by the adrenal gland in response to adrenocorticotropic hormone. It's secreted with a circadian periodicity, and peaks just before waking in the morning. It is frequently elevated in major depressive disease, certain kinds of hypertension, stress, AIDS, and at the visceral fat of obese people.
Cortisol can be measured in several matrices like urine, blood, and saliva. There's generally a very good correlation between cortisol levels in serum and saliva. The Individual Cortisol ELISA is used to measure Cortisol in human serum, plasma, urine, saliva, or cell culture medium. You can know more about Cortisol ELISA at https://www.bosterbio.com/human-cortisol-elisa-kit-ek7002-boster.html.
The Cortisol ELISA research use only one kit which was made to quantitatively measure cortisol within dried botanical extracts, spit, urine, serum, plasma, and tissue culture media trials. Complete cortisol is measured in extracted samples and in both plasma and serum, and free cortisol in urine and saliva.
A cortisol level is supplied to create a standard curve for the assay and samples have been read off a user-generated regular curve. Standard or diluted samples are placed into a transparent microtiter plate coated with an antibody to catch mouse antibodies. A cortisol peroxidase conjugate is added to the colonies.
The binding reaction is initiated with the addition of a monoclonal antibody to cortisol. Followed by an hour of incubation the plate is washed and the substrate is added. The substrate reacts with the jump cortisol-peroxidase conjugate. After a brief incubation, the reaction is stopped and the strength of the generated color is detected at a microtiter plate. Cortisol is the most potent glucocorticoid made by the adrenal gland.